PCR LAB REPORT
By:Landon Antonio PURPOSE The Purpose of the PCR lab is to learn how to use and conduct PCR with DNA. Our class used or tried to use Alu repeats within our DNA. It also leads to finding out whether we have homo or heterozygous DNA. HYPOTHESIS If the lab turns out well and is performed correctly it will show if I have homozygous DNA PROCEDURERefer to packet for procedure DATAThe 2% agarose gel ran at 150v for 20 minutes and stained using gelred for 72 hours. Lines A1 and B1 have 100bp ladder. Lines A2-7 and B2-7 have 50ml of DNA and 10ul of loading dye solution. Line B3 was my DNA solution. Observations
AnalysisSo the data above is fake because my results or my team's results were inconclusive, as were much of the class’s results too. Not enough DNA was showing if any at all so we didn’t have enough results. Errors were a very common thing in the lab especially for me, first of all on the first day we were getting our DNA and right after I centrifuged it I dropped it so all the DNA and the solution were scattered and not collected at the bottom of the tube, then my second attempt didn’t have enough DNA so i couldnt get results if i put the dye in it. I could tell it didn't have DNA because there was nothing but clear liquid showing and no strands. but then third time was the charm and i could see my DNA and everything but we still got no alu repeats/strands of DNA that we could see. Other small errors could have occurred like the pipette tip could’ve been contaminated through someone touching it. The lab could be done again and probably better because I have experience but it still wouldn't be perfect because I am not a professional nor are my classmates. The fake DNA calculations are above. CONCLUSIONEvery Lab will have mistakes yet you should learn and try again to not make the same mistakes instead of quitting the lab. This PCR lab might not have been perfect or in any way done correctly but we learned that we could probably do it better in the future and that failure is very common and easy to do. We expected to go in the lab on “the day” and see results some saw blobs or little bits of alu repeats but most saw a whole lot of nothing. We then thought, what went wrong, we came up with a couple of ideas, first we didn’t have crushed ice to keep DNA cool but we had cubes, second the pipette tips could have been contaminated one way or another, third some amounts of liquid could’ve not been exact or good, fourth while the DNA samples were being cooled the names got rubbed off so everybody couldn’t really find theirs unless their marks stayed on. Being safe and careful with all lab materials is more important than you think, one little mishap and you ruin the whole experiment and have to restart or give up. |
|